What are 3 changes someone could make in an experiment that could affect an enzyme?

Three changes that could affect an enzyme in an experiment include altering the temperature, changing the pH, and modifying substrate concentration. Increasing temperature can enhance enzyme activity up to a certain point before denaturation occurs. Adjusting the pH can impact the enzyme's shape and functionality, as each enzyme has an optimal pH range. Lastly, varying substrate concentration can influence the rate of reaction, as it affects the likelihood of enzyme-substrate collisions.