When an enzyme has denatured what has taken place?

When an enzyme has denatured, its three-dimensional structure is altered, often due to changes in temperature, pH, or chemical exposure. This structural change disrupts the enzyme's active site, preventing it from binding to its substrate effectively. As a result, the enzyme loses its catalytic activity, rendering it unable to facilitate biochemical reactions. Denaturation is typically irreversible, meaning the enzyme cannot regain its original function.