When you heat up an aqueous proteins solutions to 60 degrees C what level of proteins is not affected?

When heating aqueous protein solutions to 60 degrees Celsius, generally, the primary structure of proteins, which consists of the linear sequence of amino acids, remains unaffected. However, the secondary and tertiary structures may begin to denature, leading to changes in functionality. Some proteins, particularly those with high stability or heat resistance, may retain their structure and functionality even at this temperature.