Genes coding for ribosomal RNA (rRNA), particularly the 16S rRNA in prokaryotes and 18S rRNA in eukaryotes, are commonly used for establishing phylogenetic relationships due to their conserved nature and essential role in cellular function. Unlike previous methods that relied on morphological characteristics, which can be subjective and influenced by environmental factors, rRNA gene sequencing provides a more objective, molecular-based approach. This molecular data allows for the construction of phylogenetic trees that reflect evolutionary relationships more accurately, enabling better classification and understanding of biodiversity. Overall, the use of rRNA genes has revolutionized taxonomy by providing a robust framework for deciphering the evolutionary history of organisms.
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