During DNA replication, the leading strand is synthesized continuously in the 5' to 3' direction by DNA polymerase. The process begins with the unwinding of the double helix by helicase, which creates a replication fork. Primase then synthesizes a short RNA primer, which is extended by DNA polymerase III. The RNA primer is later replaced with DNA by DNA polymerase I. The process continues until the entire leading strand is replicated.
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