Why are recombinant plasmids cut with ecoRI?

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1237007

2026-05-19 14:51

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Recombinant plasmids are cut with the restriction enzyme EcoRI because it recognizes a specific palindromic DNA sequence (GAATTC) and cleaves it at precise locations, creating compatible sticky ends. This allows for the insertion of foreign DNA fragments into the plasmid, facilitating cloning and gene expression. EcoRI's ability to produce cohesive ends promotes efficient ligation of the desired DNA insert, ensuring successful recombinant DNA formation.

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